lamp1 (Developmental Studies Hybridoma Bank)
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Lamp1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+lamp1+antibody/anti-LAMP-1/pmc13126347-320-17-19
Average 96 stars, based on 201 article reviews
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Incubation:Article Title: Skeletal muscle TFEB signaling promotes central nervous system function and reduces neuroinflammation during aging and neurodegenerative disease. Article Snippet: For brain tissue, Fifty or 25 mg of homogenized proteins were loaded per lane, and after running Any KD, 10%, or 4–15% Mini-PROTEAN TGX Gels (BioRad, 4568124, 4561034, and 4561084), samples were transferred to 0.45 mm PVDF membranes (BioRad, 1704275), which were blocked in 5% BSA in PBS at RT for 1 h. For skeletal muscle, proteins were separated on 4–20% Stain-Free TGX gels (Bio-Rad, 5678092), separated to PVDF, and blocked in 5% non-fat dry milk in TBST (1X TBS with 0.05% Tween 20) for 1 h at RT. .. Membranes were incubated with antiFLAG antibody (Sigma, M2, F1804, 1:1000), anti-TFEB antibody (Cell Signaling, 4240,1:1000), Article Title: Specific interaction between Group B Streptococcus CC17 hypervirulent clone and phagocytes. Article Snippet: Streptococcus agalactiae also named Group B Streptococcus (GBS) is the most significant pathogen causing invasive infections, such as bacteremia and meningitis, in neonates.. Worldwide epidemiological studies have shown that a particular clonal complex (CC) of capsular serotype III, the CC17, is strongly associated with meningi tis in neonates and is therefore, designated as the hypervirulent clone.. Macrophages are a permissive niche for intracellular bacteria of all GBS clones. Article Title: Specific interaction between Group B Streptococcus CC17 hypervirulent clone and phagocytes Article Snippet: .. Following fixation, samples were permeabilized and saturated with PBS containing 0.2% saponin and 10% serum for 1 h at room temperature and then incubated for at least 1 h at room temperature with the primary Article Title: GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin Article Snippet: .. Cells were incubated with an Article Title: Restorative macrophage-derived RNAseT2 stimulates muscle stem cell fusion via an SLK/N-WASP/actin bundling dependent axis. Article Snippet: .. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX100, washed in 1X PBS and incubated overnight at 4°C with primary antibodies (antiKi67 [1:100 Abcam #ab15580], anti-myogenin [1:50 BD Pharmingen #556358]; anti-desmin [1:200 Abcam #ab32362], anti-CD206 [1:200 Abcam #ab64693], anti-phospho-NWASP [1:200 MerckMillipore #AB1966], anti-phospho-Paxillin [1:100 Sigma-Aldrich #SAB4301321], anti-HisTag [1:1000 Abcam # ab18184], anti-EEA1 antibody [1:1000 BD Biosciences #610456], anti-Rab7 antibody [1:100 CellSignaling #9367], other:Article Title: Myelin-Axon Interface Vulnerability in Alzheimer’s Disease Revealed by Subcellular Proteomics and Imaging of Human and Mouse Brain Article Snippet: |

